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青海快3跨度走势图:超敏ECL化学发光液

产品编号 产品名称 规格 价格(元) 说明书
M2301 超敏ECL化学发光液 50ml/500ml 300/2,000
Western Blot结果的好坏与否与ECL发光液的选择有着密切关系,好的ECL发光液不仅可曝光得到漂亮的目的条带,还可节省昂贵的抗体。?;?strong class="fhnd">ECL发光液用于Western、Northern和Southern blot分子杂交的化学发光检测,原理是采用新型发光底物(Luminol),其与辣根过氧化物酶反应时产生很强的发光信号,以此检测微量蛋白而获得理想的实验结果。比传统化学显色法灵敏度高,可达pg水平,发光持续时间长,信号30min内不会发生明显降低。 超敏ECL发光液

组分

组分名称 数量 保存
Super ECL A 25 ml 4℃
Super ECL B 25 ml 4℃

应用

在Western Blot印迹分析,核酸杂交以及EMSA实验中,HRP标记的二抗或探针与靶分子结合再与底物反应产生可见光。

储存

2-8°C,Super ECL A要求避光保存。

实例

超敏ECL发光液   ECL发光液
Fig1.检测Hela细胞裂解物的GAPDH蛋白,应用超敏ECL发光液(HaiGene,M2301)显色,一抗为兔抗GAPDH多克隆抗体,稀释比例为1:1000。   Fig2.检测A549细胞裂解物的AKT蛋白,应用超敏ECL发光液(HaiGene,M2301)和Competitor P公司的ECL发光液分别显色,
一抗为AKT rabbit mab(CST,#4691),稀释比例为1:1000。

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